Response of methylenetetrahydrofolate levels to methotrexate in Krebs ascites cells
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چکیده
منابع مشابه
Response of methylenetetrahydrofolate levels to methotrexate in Krebs ascites cells.
Levels of methylenetetrahydrofolate in Krebs ascites cells subsequent to transplantation and the effects of methotrexate on these levels have been measured. To directly measure methylenetetrahydrofolate in tissue extracts, the cofactor was incorporated into a covalent ternary complex with thymidylate synthase and 3H-labelled fluorodeoxyuridine monophosphate. A 3-4-day lag preceded rapid growth ...
متن کاملAltered expression of methylenetetrahydrofolate reductase modifies response to methotrexate in mice.
OBJECTIVE Folates provide one-carbon units for nucleotide synthesis and methylation reactions. A common polymorphism (677C-->T) in methylenetetrahydrofolate reductase (MTHFR) encodes an enzyme with reduced activity. Response to the antifolate methotrexate (MTX) may be modified in 677TT individuals because MTHFR converts nonmethylated folates, used for thymidine and purine synthesis, to 5-methyl...
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Histidinol is an amino acid analogue that competitively inhibits attachment of histidine to its tRNA. It produces effects on polyribosomal structure and function in normal human fibroblasts and in HeLa cells analogous to those produced in such cells by deprivation for single amino acids (Vaughan & Hansen, 1973). The inhibition of protein synthesis caused by single essential amino acids or by th...
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Two types of RNases H, which selectively degrade the RNA strand of an RNA-DNA hybrid and are inhibited by ethidium bromide, have been separated and partially purified from Krebs ascites cells by a combination of gel filtration and chromatographies on DEAE and phosphocellulose. Neither one of the two types of RNase H had any detectable activity against doublestranded RNA. These two activities we...
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Several attempts in this laboratory in the last @ or 3 years to culture the Knebs-@ ascites carcinoma of mice had failed. The maximum survival time of the cells in culture was about 10 days. The media used always contained chicken embryo ex tract (CEE). Later, during efforts to develop a culture medium and technic by which single cell cultures could readily be initiated for single-cell irradiat...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1983
ISSN: 0306-3283
DOI: 10.1042/bj2160295